We studied seasonal variation in intersexual dietary overlap, perch use, and reproductive status of female Gonatodes humeralis in Eastern Brazilian Amazon. The diet of G. humeralis consisted primarily of invertebrates, and differed significantly between sexes in the rainy season, when there was also a significant intersexual difference in perch use (males perched higher). Reproductive activity was also more intense during the rainy season. Intersexual diet divergence may be a result of differential perch use, which appears related to intense reproductive activity in the rainy season. By using higher perches, males should increase their chance to find mates as suggested in other lizard species.